Composite

Part:BBa_K3241040:Design

Designed by: Ruxiang Wang   Group: iGEM19_HUBU-WUHAN   (2019-10-16)


Pgap-PhbC-PhbA-PhbB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 465
    Illegal NheI site found at 1341
    Illegal NheI site found at 3257
    Illegal NotI site found at 2
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 495
    Illegal NgoMIV site found at 2356
    Illegal NgoMIV site found at 2441
    Illegal NgoMIV site found at 2846
    Illegal NgoMIV site found at 2975
    Illegal AgeI site found at 600
    Illegal AgeI site found at 3379
    Illegal AgeI site found at 4051
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 421
    Illegal BsaI site found at 1142
    Illegal BsaI site found at 2272


Design Notes

No


Source

PhbA PhbB PhbC are originally from Ralstonia eutropha.GenScript synthesized these genes and optimized their codons for us.

References